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Platinum Retroviral Expression Systems
Retroviral vectors are useful for delivering genes of
interest into a host cell where integration into the ge-
nome is desired. However, traditional retroviral ex-
pression technologies usually result in low viral titers,
making gene expression studies difficult.
Our Platinum Retroviral Expression Systems incorpo-
rate superior packaging cell lines and vector technolo-
gies to produce high-titer virus with a single plasmid
transfection. Each Platinum Expression System in-
cludes one of our exclusive Platinum Packaging Cell
Lines which contain the gag and pol genes. In the
Ecotropic and Amphotropic systems, the packaging
cells also contain the envelope protein. Simply clone
your gene of interest into the vector provided and
transfect into the Platinum cells. If you choose a Pan-
tropic system, simply co-transfect with the VSV-G
plasmid provided.
Not sure which Platinum Expression System is right
for you? See the previous page for a selection guide
based on the host species of your target cell.
• Higher Viral Yields: Average titer 107 infec-
tious units/mL with transient transfection
• Versatile: 3 Packaging cell lines for use with
nearly any target host species
VIRAL EXPRESSION
Retrovirus Production Using the Platinum Retroviral Expres-
sion Systems.
Are you working with stem cells? See page 24 for our Platinum Retroviral Expression Sys-
tems specifically designed for use with ES, EC and hematopoietic stem cells.
Catalog Number
VPK-300
VPK-301
Packaging Cell Line
Plat-E (Ecotropic)
Plat-A (Amphotropic)
Shuttle Vector
pMXs-Puro
pMXs-Puro
Envelope Vector
———
———
Control Vector
pMX-GFP
pMX-GFP
VPK-302
Plat-GP (Pantropic)
pMXs-Puro
pCMV-VSV-G pMX-GFP
Components of the Platinum Retroviral Expression Systems.
Product Name
Platinum Retroviral Expression System, Ecotropic
Platinum Retroviral Expression System, Amphotropic
Platinum Retroviral Expression System, Pantropic
Size
1 kit
1 kit
1 kit
Catalog Number
VPK-300
VPK-301
VPK-302