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1. Human sICAM-2/CD102 ELISA KIT Intended use
The Human sICAM-2 ELISA is to be used for the in-vitro quantitative determination of soluble Inter Cellular Adhesion Molecule -2 (sICAM-2) in human serum, plasma, buffered solutions or cell culture medium. The assay will recognize both natural and recombinant human sICAM-2 This kit has been configured for research use only.
2. Human sICAM-2/CD102 ELISA KIT Principle of the method
The sICAM-2 /CD102 Kit is a solid phase sandwich Enzyme linked-Immuno- Sorbent Assay (ELISA). A monoclonal antibody specific for ICAM-2 has been coated onto the wells of the microtiter strips provided.Samples, including standards of known sICAM-2 concentrations, control specimens and unknowns are pipetted into these wells.During the first incubation, the sICAM-2 antigen and a biotinylated monoclonal antibody specific for ICAM-2 are simultaneously incubated.After washing, the enzyme ( streptavidin-peroxydase ) is added. After incubation and washing to remove all the unbound enzyme, a substrate solution which is acting on the bound enzyme is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentratio of sICAM-2 present in the samples.
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The Human sICAM-2 ELISA is to be used for the in-vitro quantitative determination of soluble Inter Cellular Adhesion Molecule -2 (sICAM-2) in human serum, plasma, buffered solutions or cell culture medium. The assay will recognize both natural and recombinant human sICAM-2 This kit has been configured for research use only.
2. Human sICAM-2/CD102 ELISA KIT Principle of the method
The sICAM-2 /CD102 Kit is a solid phase sandwich Enzyme linked-Immuno- Sorbent Assay (ELISA). A monoclonal antibody specific for ICAM-2 has been coated onto the wells of the microtiter strips provided.Samples, including standards of known sICAM-2 concentrations, control specimens and unknowns are pipetted into these wells.During the first incubation, the sICAM-2 antigen and a biotinylated monoclonal antibody specific for ICAM-2 are simultaneously incubated.After washing, the enzyme ( streptavidin-peroxydase ) is added. After incubation and washing to remove all the unbound enzyme, a substrate solution which is acting on the bound enzyme is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentratio of sICAM-2 present in the samples.
网址：http://www.craymeibio.com/