发送询价
通用名称：小鼠黄体生成素LH ELISA试剂盒英文名称：Mouse LH Elisa kit
类型：激素检测系列
1. Mouse LH Elisa kit INTENDED USE
This BG LH ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse LH. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
2. Mouse LH Elisa kit PRINCIPLE OF THE ASSAY
LH ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-LH antibody and an LH-HRP conjugate. The assay sample and buffer are incubated together with LH-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the LH concentration since LH from samples and LH-HRP conjugate compete for the anti-LH antibody binding site. Since the number of sites is limited, as more sites are occupied by LH from the sample,fewer sites are left to bind LH-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The LH concentration in each sample is interpolated from this standard curve.
网址：http://www.craymeibio.com/