发送询价
通用名称：小鼠雌二醇E2 ELISA试剂盒英文名称：Mouse Estradiol (E2) Elisa kit
简介：
1. Mouse Estradiol (E2) Elisa kit INTENDED USE
This BG E2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative
determination of Mouse E2. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
2. Mouse Estradiol (E2) Elisa kit PRINCIPLE OF THE ASSAY
E2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti- E2 antibody and an E2-HRP conjugate. The assay sample and buffer are incubated together with E2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the E2 concentration since E2 from samples and E2-HRP conjugate compete for the anti-E2 antibody binding site. Since the number of sites is limited, as more sites are occupied by E2 from the sample, fewer sites are left to bind
E2-HRP conjugate. A standard curve is plotted relating the intensity of the color(O.D.) to the concentration of standards. The E2 concentration in each sample is
interpolated from this standard curve.
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