发送询价
通用名称：小鼠孕酮ELISA试剂盒英文名称：Mouse Progesterone (PROG) Elisa kit
1. Mouse Progesterone (PROG) Elisa kit INTENDED USE
This BG PROG ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse PROG. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
2. Mouse Progesterone (PROG) Elisa kit PRINCIPLE OF THE ASSAY
PROG ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PROG antibody and an PROG-HRP conjugate. The assay sample and buffer are incubated together with PROG-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times.
The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PROG concentration since PROG from samples and PROG-HRP conjugate compete for the anti-PROG antibody binding site. Since the number of sites is limited, as more sites are occupied by PROG from the sample, fewer sites are left to bind PROG-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards.
The PROG concentration in each sample is interpolated from this standard curve.
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