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Triquick Reagent 总RNA提取试剂 产品简介：本公司生产的TRIquick总RNA提取试剂对细菌和动植 物组织细胞中的RNA提取均适用，在样品裂解或匀浆的过程中， TRIquick可以保持RNA的完整性，同时裂解细胞，溶解细胞内含 物。加入之后，溶液分为水相和有机相，RNA在水相中。取 出水相即可用异丙醇沉淀回收RNA；中间层用乙醇沉淀可以回收 DNA；有机相用异丙醇沉淀可以回收蛋白质。该试剂既可用于小 量样品(50-100mg组织或5×106细胞)，也可用于大量样品(≥1g组 织或≥107细胞)。流程简单快速，一个小时内即可完成实验。提 取的总RNA没有DNA和蛋白的污染，可用于RT-PCR、Northern blot、Dot Blot、mRNA提取、cDNA合成、polyA 筛选、体外翻译 和RNase 保护分析等试验。 产品特点：本公司生产的TRIquick为红色，便于区分水相和有机 相，方便使用。 保存要求：4℃避光保存，保质期12个月。 注意事项： 1、常更换新手套，防止皮肤表面含有的RNase导致污染。 2、操作过程中使用的无RNase的塑料制品和枪头以避免交叉污染。 3、配制溶液应使用无RNase的水。 4、TRIquick有较强的腐蚀性，应尽量避免皮肤接触或吸入体内。
Triquick Reagent 总RNA提取试剂进口
TriQuick Reagent
Catalog Numbers: R1100 Quantity :100ml Storage: 4℃,Keep in Dark Place Shelflife: 12 Triquick Reagent 总RNA提取试剂进口 Product Information: TriQuick Reagent is a complete, ready-to-use reagent for the isolation of high-quality total RNA from cell and tissue samples of animal, plant, yeast, or bacterial origin. It maintains the integrity of the RNA due to highly effective inhibition of RNase activity while disrupting cells. The RNA can be used in downstream applications such as Northern blotting, mRNA purification, Invitro-trans Reagent performs well with small quantities of tissue (50-100 mg) and cells (5 × 10 6 ) for 100 times. Operating Instructions:Triquick Reagent 总RNA提取试剂进口 Materials Needed( not supplied): Chloroform, Isopropyl alcohol, 75% ethanol, RNase-free water 1. Samples preparation ： 1) Adherent Cells：Remove growth media from culture dish. Add 1 mL TriQuick Reagent directly to the cells in the culture dish per 10 cm 2 of culture dish surface area. (Note: Add 1 mL TriQuick Reagent for a 35 mm dish, 3 mL for a 60 mm dish, and 8 mL for a 100 mm dish.) Lyse the cells directly in the culture dish by pipetting the cells up and down several times. 2) Suspension Cells：Harvest cells by centrifugation and remove media. Add 1 mL of TriQuick Reagent to 5× 10 6 –1 ×10 7 cells from animal, plant or yeast origin, or 1 × 10 7 cells of bacterial origin. Lyse cells in sample by pipetting up and down several times. Disruption of some yeast and bacterial cells may require the use of a homogenizer. 3) Tissues：Add 1 mL TriQuick Reagent per 50–100 mg of tissue sample. Homogenize sample using a glass or power homogenizer. （Note: Process or freeze tissue samples immediately upon collection.） Incubate the lysing sample for 5 minutes at room temperature to permit complete dissociation of the nucleoprotein complex . For samples with high content of proteins, polysaccharides, or extracellular material, an additional centrifugation at 12,000 × g for 10 minutes at 4°C may be required to remove insoluble material from the samples. Transfer the cleared supernatant to a new tube.Triquick Reagent 总RNA提取试剂进口 2. Phase separation Add 0.2 mL of chloroform per 1 mL of TriQuick Reagent used for homogenization. Cap the tube securely. Shake tube vigorously for 30 seconds. Incubate for 2–3 minutes at room temperature. Centrifuge the sample at 12,000×g for 10 minutes at 4°C. pipetting the aqueous phase into a new tube. Avoid drawing any of the interphase or organic layer into the pipette when removing the aqueous phase. 3. RNA precipitation Add 0.5 mL of isopropanol to the aqueous phase, per 1 mL of TriQuick Reagent used for homogenization. Incubate at room temperature for 10 minutes. Centrifuge at 12,000 ×g for 10 minutes at 4°C. Remove the supernatant from the tube, leaving only the RNA pellet. Wash the pellet with 1 mL of 75% ethanol.Vortex the sample briefly, then centrifuge the tube at 12000×g for 2minutes at 4°C. Discard the wash. Vacuum or air dry the RNA pellet for 5–10 minutes. 4. RNA resuspension Resuspend the RNA pellet in RNase-free water (20–50 μL), Proceed to downstream application, or store at –70°C. Caution 1, The Environment and all equipments used in experiment like tubes, pipets should be Rnase-free to aviod RNA degradation. 2, Always work with TriQuick Reagent in a fume hood, and always wear a lab coat, gloves and safety glasses. Avoid direct contact with TriQuick Reagent Related products ： R1600 DEPC treated water P1011 phenol:chloroform: isopropanol ＝ 25 ： 24 ： 1 （ PH<5.0 ） R1050 5×RNA Loading Buffer M1010 10×MOPS buffer SR0020 RNAwaitTriQuick Reagent Catalog Numbers: R1100 Quantity :100ml Storage: 4℃,Keep in Dark Place Shelflife: 12 months Product Information: TriQuick Reagent is a complete, ready-to-use reagent for the isolation of high-quality total RNA from cell and tissue samples of animal, plant, yeast, or bacterial origin. It maintains the integrity of the RNA due to highly effective inhibition of RNase activity while disrupting cells. The RNA can be used in downstream applications such as Northern blotting, mRNA purification, Invitro-trans Reagent performs well with small quantities of tissue (50-100 mg) and cells (5 × 10 6 ) for 100 times. Operating Instructions: Materials Needed( not supplied): Chloroform, Isopropyl alcohol, 75% ethanol, RNase-free water 1. Samples preparation ： 1) Adherent Cells：Remove growth media from culture dish. Add 1 mL TriQuick Reagent directly to the cells in the culture dish per 10 cm 2 of culture dish surface area. (Note: Add 1 mL TriQuick Reagent for a 35 mm dish, 3 mL for a 60 mm dish, and 8 mL for a 100 mm dish.) Lyse the cells directly in the culture dish by pipetting the cells up and down several times. 2) Suspension Cells：Harvest cells by centrifugation and remove media. Add 1 mL of TriQuick Reagent to 5× 10 6 –1 ×10 7 cells from animal, plant or yeast origin, or 1 × 10 7 cells of bacterial origin. Lyse cells in sample by pipetting up and down several times. Disruption of some yeast and bacterial cells may require the use of a homogenizer. 3) Tissues：Add 1 mL TriQuick Reagent per 50–100 mg of tissue sample. Homogenize sample using a glass or power homogenizer. （Note: Process or freeze tissue samples immediately upon collection.） Incubate the lysing sample for 5 minutes at room temperature to permit complete dissociation of the nucleoprotein complex . For samples with high content of proteins, polysaccharides, or extracellular material, an additional centrifugation at 12,000 × g for 10 minutes at 4°C may be required to remove insoluble material from the samples. Transfer the cleared supernatant to a new tube. 2. Phase separation Add 0.2 mL of chloroform per 1 mL of TriQuick Reagent used for homogenization. Cap the tube securely. Shake tube vigorously for 30 seconds. Incubate for 2–3 minutes at room temperature. Centrifuge the sample at 12,000×g for 10 minutes at 4°C. pipetting the aqueous phase into a new tube. Avoid drawing any of the interphase or organic layer into the pipette when removing the aqueous phase. 3. RNA precipitation Add 0.5 mL of isopropanol to the aqueous phase, per 1 mL of TriQuick Reagent used for homogenization. Incubate at room temperature for 10 minutes. Centrifuge at 12,000 ×g for 10 minutes at 4°C. Remove the supernatant from the tube, leaving only the RNA pellet. Wash the pellet with 1 mL of 75% ethanol.Vortex the sample briefly, then centrifuge the tube at 12000×g for 2minutes at 4°C. Discard the wash. Vacuum or air dry the RNA pellet for 5–10 minutes. 4. RNA resuspension Resuspend the RNA pellet in RNase-free water (20–50 μL), Proceed to downstream application, or store at –70°C. Caution 1, The Environment and all equipments used in experiment like tubes, pipets should be Rnase-free to aviod RNA degradation. 2, Always work with TriQuick Reagent in a fume hood, and always wear a lab coat, gloves and safety glasses. Avoid direct contact with TriQuick Reagent Related products ：Triquick Reagent 总RNA提取试剂进口 R1600 DEPC treated water P1011 phenol:chloroform: isopropanol ＝ 25 ： 24 ： 1 （ PH<5.0 ） R1050 5×RNA Loading Buffer M1010 10×MOPS buffer SR0020 RNAwait |