Standard program* of columns packed with NUCLEODUR? C18 Gravity

Column system

粒径:

1.8 μm

3 μm

5 μm

10 μm

分析柱

EC columns (standard system, 2–4.6 mm)

SHAPE \* MERGEFORMAT

SHAPE \* MERGEFORMAT

+

SHAPE \* MERGEFORMAT

+

制备

VarioPrep columns (VP, ≥ 8 mm)

SHAPE \* MERGEFORMAT

SHAPE \* MERGEFORMAT

+

Length ->

30 mm

50 mm

75 mm

100 mm

125 mm

150 mm

250 mm

NUCLEODUR? C18 Gravity, 1.8 μm

2 mm ID

760078.20

760079.20

760071.20

760076.20

760075.20

3 mm ID

760078.30

760079.30

760076.30

4 mm ID

760078.40

760079.40

760076.40

4.6 mm ID

760078.46

760079.46

760076.46

EC guard columns*

4 x 2 mm

761901.20

4 x 3 mm

761901.30

NUCLEODUR? C18 Gravity, 3 μm

2 mm ID

760080.20

760084.20

760081.20

760083.20

760082.20

3 mm ID

760080.30

760084.30

760081.30

760083.30

760082.30

4 mm ID

760080.40

760084.40

760081.40

760083.40

760082.40

4.6 mm ID

760080.46

760086.46

760084.46

760081.46

760083.46

760082.46

EC guard columns*

4 x 2 mm

761902.20

4 x 3 mm

761902.30

NUCLEODUR? C18 Gravity, 5 μm

2 mm ID

760102.20

760104.20

760100.20

760103.20

760101.20

3 mm ID

760102.30

760104.30

760100.30

760103.30

760101.30

4 mm ID

760102.40

760104.40

760100.40

760103.40

760101.40

4.6 mm ID

760102.46

760106.46

760104.46

760100.46

760103.46

760101.46

EC guard columns*

4 x 2 mm

761903.20

4 x 3 mm

761903.30

Guard columns for EC columns

EC columns with ID

Use guard 
column holder

2 mm

3 mm

4 mm

4.6 mm

*

Column Protection System

EC guard column

4/2

4/3

4/3

4/3

REF 718966

Length

10 mm ID

21 mm ID

32 mm ID

40 mm ID

NUCLEODUR? C18 Gravity, 5 μm

50 mm

762103.100

762103.210

125 mm

762109.100

762109.210

150 mm

762100.400

250 mm

762113.100

762113.210

762113.320

762113.400

NUCLEODUR? C18 Gravity, 10 μm

250 mm

762250.210

762250.400

VP guard columns with NUCLEODUR? C18 Gravity

8 mm ID

16 mm ID

32 mm ID

10 mm*

762160.80

762160.160

15 mm*

762163.320

* VP guard column

Size

Pack of

for VP column

required holder

REF

VP 10/8

10 x 8 mm ID

2

10 mm ID

8 mm

718251

VP 10/16

10 x 16 mm ID

2

21 mm ID

16 mm

718256

VP 15/32

15 x 32 mm ID

1

32–40 mm ID

32 mm

718253

舒巴坦钠理论塔板数

舒巴坦钠
与杂质分离度

重现性

C18 Gravity

7398

4.13

0.71

对照柱X

6317

4.00

0.15

Substances:

vancomycin

Column:

EC 250/4.6 NUCLEODUR^® C18 Gravity, 5 µm

Phase:

NUCLEODUR^® C18 Gravity

REF number:

760101.46

Sample pretreatment:

Sample concentration: 2 mg/mL in eluent A
Stock solution: 4 mL triethylamine + 1996 mL water + H₃PO₄ (adjusted to pH 3.2)

Conditions:

Eluent A: 920 mL stock solution + 10 mL THF + 70 mL ACN
Eluent B: 700 mL stock solution + 10 mL THF + 290 mL ACN
Gradient: 0 % B for 13 min, 0-100 % B in 9.1 min, 100 % B for 4 min
Flow rate: 1.0 mL/min
Temperature: 25 °C
Injection volume: 20 µL

Detection:

UV, 280 nm

Author(-s):

HPLC department

Source:

MACHEREY-NAGEL, Germany 2010

Keywords:

antibiotics; pharmacopeia; Pharm. Eu.; asymmetry factor

Legend:

1  -  vancomycin

Note:

Vancomycin is determined with an asymmetry factor of 1.12 (Pharm. Eu < 1.60)

Substances:

acetylsalicylic acid; sulindac; piroxicam; suprofen; tolmetin; naproxen; diflunisal; fenoprofen; flurbiprofen; niflumic acid; indomethacin; ibuprofen

Column:

EC 250/4.6 NUCLEODUR^® C18 Gravity, 5 µm

Phase:

NUCLEODUR^® C18 Gravity

MN catalog number:

760101.46

Conditions:

Mobile phase: acetonitrile / 20 mM KH₂PO₄, pH 2.5, 45:55 (v/v)
Flowrate: 1.3 ml/min
Temperature: 22 °C
Injection volume: 5.0 µl

Detection:

UV, 230 nm

Author(-s):

HPLC department

Source:

MACHEREY-NAGEL, Germany 2008

Keywords:

antiinflamatoray drugs; drugs; NSAID

Legend:

1   -  acetylsalicylic acid
2   -  sulindac
3   -  piroxicam
4   -  suprofen
5   -  tolmetin
6   -  naproxen
7   -  diflunisal
8   -  fenoprofen
9   -  flurbiprofen
10 -  niflumic acid
11 -  indomethacin
12 -  ibuprofen

Substances:

coumafuryl; warfarin; pindone; coumatetralyl; coumachlor; diphacinone; chlorphacinone; bromadiolone i; difenacoum; bromadiolone ii; flocoumafen i; flocumafen ii; brodifacoum; difethialone; pyranocoumarin

Column:

EC 250/4.6 NUCLEODUR^® C18 Gravity, 5 μm
CC 8/4 NUCLEODUR^® C18 Gravity, 5 μm (guard column)

Phase:

NUCLEODUR^® C18 Gravity

REF number:

760101.46
761125.40

Conditions:

Buffer:
0.68 g KH₂PO₄ + 0.34 g Na₃PO₄ x 12 H₂0 in 1l dest. water, pH 6.3
Eluents:
A: Methanol / Acetonitrile / Buffer (250:100:650)
B: Methanol / Acetonitrile / Buffer (100:650:250)
Flow rate: 1.2 ml/min
Temperature: RT

Detection:

A) DAD UV, 308 nm (285 nm, 260 nm)
B) Fluorescence (stimulation 308 nm / emission 390 nm)

Author(-s):

Freidhof, K.

Source:

Landesuntersuchungsamt Rheinland-Pfalz, Koblenz; Germany

Keywords:

anti-coagulant drugs; drugs

Legend:

Peaks:

A)
1) Coumafuryl
2) Warfarin & Pindone
3) Coumatetralyl
4) Coumachlor
5) Diphacinone
6) Chlorphacinone
7) Bromadiolone I
8) Difenacoum
9) Bromadiolone II
10) Flocoumafen I
11) Flocumafen II & Brodifacoum
12) Difethialone
13) Pyranocoumarin

B)
1) Coumafuryl
2) Warfarin
3) Coumatetralyl
4) Coumachlor
5) Bromadiolone I
6) Difenacoum
7) Bromadiolone II
8) Flocoumafen & Brodifacoum
9) Pyranocoumarin